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human rh2a polyclonal antibody  (Proteintech)


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    Proteintech human rh2a polyclonal antibody
    Fig. 3. Microscopic image and growth curve of HEK293 WT and <t>RH2A-KO</t> cells.
    Human Rh2a Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+rh2a+polyclonal+antibody/RNASEH2A+Antibody/10__14533_slash_jbm__24__33-66-75-81
    Average 93 stars, based on 25 article reviews
    human rh2a polyclonal antibody - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Generation of ribonuclease H2 A subunit (RH2A)-knockout HEK293 cells and analysis of the ribonucleotide content of their genomic DNA"

    Article Title: Generation of ribonuclease H2 A subunit (RH2A)-knockout HEK293 cells and analysis of the ribonucleotide content of their genomic DNA

    Journal: Journal of Biological Macromolecules

    doi: 10.14533/jbm.24.33

    Fig. 3. Microscopic image and growth curve of HEK293 WT and RH2A-KO cells.
    Figure Legend Snippet: Fig. 3. Microscopic image and growth curve of HEK293 WT and RH2A-KO cells.

    Techniques Used:

    Fig. 5. Expression of RH2A.
    Figure Legend Snippet: Fig. 5. Expression of RH2A.

    Techniques Used: Expressing

    Fig. 6. Expression of RH2A variants with AGS- causing mutation in RH2A-KO cells.
    Figure Legend Snippet: Fig. 6. Expression of RH2A variants with AGS- causing mutation in RH2A-KO cells.

    Techniques Used: Expressing, Mutagenesis

    Related Articles

    Membrane:

    Article Title: Generation of ribonuclease H2 A subunit (RH2A)-knockout HEK293 cells and analysis of the ribonucleotide content of their genomic DNA
    Article Snippet: The solution (20 μL) was applied to 12.5% w/v SDSpolyacrylamide gel and was run at 40 mA for 40 min. Pre-stained Protein Markers (Nacalai Tesque) consisting of β-galactosidase (112 kDa), bovine serum albumin (87 kDa), glutamine dehydrogenase (59 kDa), ovalbumin (47 kDa), carbonic anhydrase (33 kDa), myoglobin (27 kDa), and lysozyme (20 kDa) was used for marker proteins. .. After separation, the proteins were transferred by electroblotting onto a polyvinylidene difluoride (PVDF) membrane Sequi- BlotTM PVDF (BioRad, Hercules, CA) in 25 mM Tris-HCl buffer (pH 8.3), 192 mM glycine, 20% v/v methanol at 25 V for 50 min. After blotting, the membrane was washed with 50 mM Tris-HCl buffer (pH 8.3), 138 mM NaCl, 2.7 mM KCl, 0.05% Tween 20 (TBS-T), blocked with TBS-T containing 2% w/v skim milk, and incubated with mouse anti human RH2A polyclonal antibody, Anti RNASEH2A (Proteintech, Rosemont, IL, 1:1000 in TBS-T containing 1% w/v skim milk). .. Then, the membrane was incubated with HRP-conjugated goat anti-rabbit immunoglobulins (Agilent Technologies, Santa Clara, CA, 1:1000 in TBS-T containing 1% w/v skim milk).

    Incubation:

    Article Title: Generation of ribonuclease H2 A subunit (RH2A)-knockout HEK293 cells and analysis of the ribonucleotide content of their genomic DNA
    Article Snippet: The solution (20 μL) was applied to 12.5% w/v SDSpolyacrylamide gel and was run at 40 mA for 40 min. Pre-stained Protein Markers (Nacalai Tesque) consisting of β-galactosidase (112 kDa), bovine serum albumin (87 kDa), glutamine dehydrogenase (59 kDa), ovalbumin (47 kDa), carbonic anhydrase (33 kDa), myoglobin (27 kDa), and lysozyme (20 kDa) was used for marker proteins. .. After separation, the proteins were transferred by electroblotting onto a polyvinylidene difluoride (PVDF) membrane Sequi- BlotTM PVDF (BioRad, Hercules, CA) in 25 mM Tris-HCl buffer (pH 8.3), 192 mM glycine, 20% v/v methanol at 25 V for 50 min. After blotting, the membrane was washed with 50 mM Tris-HCl buffer (pH 8.3), 138 mM NaCl, 2.7 mM KCl, 0.05% Tween 20 (TBS-T), blocked with TBS-T containing 2% w/v skim milk, and incubated with mouse anti human RH2A polyclonal antibody, Anti RNASEH2A (Proteintech, Rosemont, IL, 1:1000 in TBS-T containing 1% w/v skim milk). .. Then, the membrane was incubated with HRP-conjugated goat anti-rabbit immunoglobulins (Agilent Technologies, Santa Clara, CA, 1:1000 in TBS-T containing 1% w/v skim milk).



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    human rh2a polyclonal antibody  (Proteintech)
    93
    Proteintech human rh2a polyclonal antibody
    Fig. 3. Microscopic image and growth curve of HEK293 WT and <t>RH2A-KO</t> cells.
    Human Rh2a Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+rh2a+polyclonal+antibody/RNASEH2A+Antibody/10__14533_slash_jbm__24__33-66-75-81
    Average 93 stars, based on 1 article reviews
    human rh2a polyclonal antibody - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 3. Microscopic image and growth curve of HEK293 WT and RH2A-KO cells.

    Journal: Journal of Biological Macromolecules

    Article Title: Generation of ribonuclease H2 A subunit (RH2A)-knockout HEK293 cells and analysis of the ribonucleotide content of their genomic DNA

    doi: 10.14533/jbm.24.33

    Figure Lengend Snippet: Fig. 3. Microscopic image and growth curve of HEK293 WT and RH2A-KO cells.

    Article Snippet: After separation, the proteins were transferred by electroblotting onto a polyvinylidene difluoride (PVDF) membrane Sequi- BlotTM PVDF (BioRad, Hercules, CA) in 25 mM Tris-HCl buffer (pH 8.3), 192 mM glycine, 20% v/v methanol at 25 V for 50 min. After blotting, the membrane was washed with 50 mM Tris-HCl buffer (pH 8.3), 138 mM NaCl, 2.7 mM KCl, 0.05% Tween 20 (TBS-T), blocked with TBS-T containing 2% w/v skim milk, and incubated with mouse anti human RH2A polyclonal antibody, Anti RNASEH2A (Proteintech, Rosemont, IL, 1:1000 in TBS-T containing 1% w/v skim milk).

    Techniques:

    Fig. 5. Expression of RH2A.

    Journal: Journal of Biological Macromolecules

    Article Title: Generation of ribonuclease H2 A subunit (RH2A)-knockout HEK293 cells and analysis of the ribonucleotide content of their genomic DNA

    doi: 10.14533/jbm.24.33

    Figure Lengend Snippet: Fig. 5. Expression of RH2A.

    Article Snippet: After separation, the proteins were transferred by electroblotting onto a polyvinylidene difluoride (PVDF) membrane Sequi- BlotTM PVDF (BioRad, Hercules, CA) in 25 mM Tris-HCl buffer (pH 8.3), 192 mM glycine, 20% v/v methanol at 25 V for 50 min. After blotting, the membrane was washed with 50 mM Tris-HCl buffer (pH 8.3), 138 mM NaCl, 2.7 mM KCl, 0.05% Tween 20 (TBS-T), blocked with TBS-T containing 2% w/v skim milk, and incubated with mouse anti human RH2A polyclonal antibody, Anti RNASEH2A (Proteintech, Rosemont, IL, 1:1000 in TBS-T containing 1% w/v skim milk).

    Techniques: Expressing

    Fig. 6. Expression of RH2A variants with AGS- causing mutation in RH2A-KO cells.

    Journal: Journal of Biological Macromolecules

    Article Title: Generation of ribonuclease H2 A subunit (RH2A)-knockout HEK293 cells and analysis of the ribonucleotide content of their genomic DNA

    doi: 10.14533/jbm.24.33

    Figure Lengend Snippet: Fig. 6. Expression of RH2A variants with AGS- causing mutation in RH2A-KO cells.

    Article Snippet: After separation, the proteins were transferred by electroblotting onto a polyvinylidene difluoride (PVDF) membrane Sequi- BlotTM PVDF (BioRad, Hercules, CA) in 25 mM Tris-HCl buffer (pH 8.3), 192 mM glycine, 20% v/v methanol at 25 V for 50 min. After blotting, the membrane was washed with 50 mM Tris-HCl buffer (pH 8.3), 138 mM NaCl, 2.7 mM KCl, 0.05% Tween 20 (TBS-T), blocked with TBS-T containing 2% w/v skim milk, and incubated with mouse anti human RH2A polyclonal antibody, Anti RNASEH2A (Proteintech, Rosemont, IL, 1:1000 in TBS-T containing 1% w/v skim milk).

    Techniques: Expressing, Mutagenesis